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Title:Electron cryo-microscopy of ABC BmrA in 12-fold symmetry rings
Authors:Fribourg PF, Chami M, Sorzano CO, Gubellini F, Marabini R, Marco S, Jault JM, Levy D
Sample:BmrA reconstituted at high density into lipid bilayer.
Method:Single particle reconstruction (23 angstroms resolution)
Red flagLatest update:2014-05-14
Sample
Sample name: BmrA reconstituted at high density into lipid bilayer.
Oligomeric state: 24 Homodimers of BmrA inserted into lipid bilayer
Experimental molecular weight of the sample: 3.12
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1proteinBmrA3.123.12DimertrueBacillus subtilis24 homodimers of inserted into lipid bilayer
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.80.1 mg/mL50mM HEPES, 100mM NaClTed Pella inc. holey formvar grid
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KHOMEMADE PLUNGERBlot for 3 seconds before plunging. msspecimen linked to a Ni++-NTA-DOGS/DOPC lipid monolayer
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM200FEG200 kVFLOOD BEAMBRIGHT FIELD2.26 mm2600 nm5000 nm5000050000FIELD EMISSION GUNKODAK SO-163 FILM mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
GATAN LIQUID NITROGEN°° eV K K K mrad15 e/Å201-JAN-2003
Processing
Protocol:Projection matching refinement
Software:Xmipp
CTF correction:Micrograph based
Number of particles:1188
Imposed symmetry:D12
Resolution by author:23 Å
Resolution method:FSC 0.5
Processing details:Xmipp CL2D classification and Projection matching refinement with Invert Fourier 3D reconstruction.
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
102 μm/pixel8NIKON COOLSCAN
Publicly available raw data:http://umr168.curie.fr/en/research-groups/structural-analysis-membrane-proteins-and-biomimetic-systems-electron-microscopy/str
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
3G61 rigid bodyCross correlation factorChimeraREALProtocol: Rigid body