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Title:Dissecting the in vivo assembly of the 30S ribosomal subunit reveals the role of RimM
Authors:Guo Q, Goto S, Chen Y, Muto A, Himeno H, Deng H, Lei J, Gao N
Sample:immature ribosomal small subunit from rimm gene deleted E.coli strain
Method:Single particle reconstruction (14.0 angstroms resolution)
Red flagLatest update:2013-03-06
Sample
Sample name: immature ribosomal small subunit from rimm gene deleted E.coli strain
Experimental molecular weight of the sample: 0.8
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1ribosome-prokaryotesmall subunit from rimm gene deleted E.coli strain0.80.8falseEscherichia coli
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.5 mg/mL150 mM NH4Cl,10mM Tris-HCL,10mM MgCl2
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE100% KFEI VITROBOT MARK IVBlot for 1 seconds before plunging ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F20200 kVFLOOD BEAMBRIGHT FIELD mm1300 nm4000 nm80000FIELD EMISSION GUNGENERIC GATAN (4k x 4k) mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
GATAN LIQUID NITROGEN°° eV K K K mrad20 e/Å201-JAN-2012
Processing
Protocol:Reference Projections
Software:Spider
CTF correction:Weiner filter
Number of particles:26670
Imposed symmetry:C1
Resolution by author:14.0 Å
Resolution method:FSC 0.5
Processing details:This is a classification volume (No. 2) using ML3D methods.
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
3OFA flexibleMDFFREALProtocol: MDFF