top_cornerHomeEMD-5500  Contact EMDataBank 
Image unavailable
Title:Dissecting the in vivo assembly of the 30S ribosomal subunit reveals the role of RimM
Authors:Guo Q, Goto S, Chen Y, Muto A, Himeno H, Deng H, Lei J, Gao N
Sample:immature ribosomal small subunit from rimm gene deleted E.coli strain
Method:Single particle reconstruction (12.9 angstroms resolution)
Red flagLatest update:2013-03-06
Sample
Sample name: immature ribosomal small subunit from rimm gene deleted E.coli strain
Experimental molecular weight of the sample: 0.8
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1ribosome-prokaryotesmall subunit from rimm gene deleted E.coli strain0.80.8falseEscherichia coli
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.5 mg/mL150 mM NH4Cl,10mM Tris-HCL,10mM MgCl2
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE100% KFEI VITROBOT MARK IVBlot for 2 seconds before plunging ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F20200 kVFLOOD BEAMBRIGHT FIELD mm1300 nm4000 nm80000FIELD EMISSION GUNGENERIC CCD mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
GATAN LIQUID NITROGEN°° eV K K K mrad20 e/Å201-JAN-2012
Processing
Protocol:Reference Projections
Software:Spider
CTF correction:Weiner filter
Number of particles:32152
Imposed symmetry:C1
Resolution by author:12.9 Å
Resolution method:FSC 0.5
Processing details:This is a classification result using ML3D methods.
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
3OFA flexibleMDFFREALProtocol: MDFF