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Title:Cryo-EM reconstruction of Sputnik virus
Authors:Zhang XZ, Sun SY, Xiang Y, Wong J, Klose T, Raoult D, Rossmann MG
Sample:Sputnik
Method:Single particle reconstruction (3.5 angstroms resolution)
Red flagLatest update:2012-11-14
Sample
Sample name: Sputnik
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1virusSputnik virophageSputnik virophageSputnik virus full particle
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
71 mg/mLPBS400 mesh Cu grid (c-flat), 1.2 um hole, glow discharged
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE90%100 KGATAN CRYOPLUNGE 3blot 6 seconds ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TITAN KRIOS300 kVFLOOD BEAMBRIGHT FIELD2.7 mm1100 nm2400 nm59000FIELD EMISSION GUNKODAK SO-163 FILM mmcorrected at 250,000 magnification

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
FEI TITAN KRIOS AUTOGRID HOLDER°° eV K K K mrad22 e/Å201-JAN-2011
Processing
Protocol:Projection matching
Software:EMAN, Frealign
CTF correction:each particle
Number of particles:12000
Imposed symmetry:I
Resolution by author:3.5 Å
Resolution method:FSC 0.143
Processing details:Using EMAN.
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
6.35 μm/pixelNIKON SUPER COOLSCAN 9000
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails