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Title:Dynamics in Cryo EM Reconstructions Visualized with Maximum-Likelihood Derived Variance Maps
Authors:Wang Q, Matsui T, Domitrovic T, Zheng Y, Doerschuk PC, Johnson JE
Sample:Cryo-EM reconstruction of fully-mature capsid of Nudaurelia capensis omega virus (NwV)
Method:Single particle reconstruction (20 angstroms resolution)
Sample
Sample name: Cryo-EM reconstruction of fully-mature capsid of Nudaurelia capensis omega virus (NwV)
Oligomeric state: Icosahedral virus
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1virusNudaurelia capensis omega virusNudaurelia capensis omega virus
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
mg/mL
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KFEI VITROBOT MARK I ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F20120 kVFLOOD BEAMBRIGHT FIELD mm nm nmFIELD EMISSION GUN mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
GATAN LIQUID NITROGEN°° eV K K K mrad e/Å213-FEB-2008
Processing
Protocol:Maximum Likelihood
CTF correction:Each image
Number of particles:1200
Imposed symmetry:I
Resolution by author:20 Å
Resolution method:FSC 0.5
Processing details:The reconstruction uses a maximum likelihood algorithm that explicitly takes into account the continuous variability from one instance to another instance of the particle.
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
μm/pixelData were collected using the Leginon automated electron microscopy package and data processing was performed using the Appion pipeline.
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails