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Title:MDA5-dsRNA filament
Authors:Berke IC, Yu X, Modis Y, Egelman EH
Sample:MDA5-dsRNA
Method:Helical reconstruction (20 angstroms resolution)
Red flagLatest update:2013-07-17
Sample
Sample name: MDA5-dsRNA
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1proteinMDA5trueMus musculus
Experiment
Specimen state: Filament
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
mg/mL
Helical parameters:
HandPhi incrementAzimuthal (Z) increment
RIGHT HANDED82.7°43.6 Å
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
NONE% KNONE ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI 1280 kVFLOOD BEAMBRIGHT FIELD mm nm nmTUNGSTEN HAIRPINKODAK SO-163 FILM mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
OTHER°° eV K K K mrad e/Å201-MAY-2012
Processing
Protocol:IHRSR
Software:Spider
Resolution by author:20 Å
Resolution method:FSC 0.5
Processing details:The particles were processed using IHRSR.
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
4.16 μm/pixel14NIKON COOLSCAN
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails