top_cornerHomeEMD-5440  Contact EMDataBank 
Image unavailable
Title:Regulation of mammalian transcription by Gdown1 through a novel steric crosstalk revealed by cryo-EM
Authors:Wu YM, Chang JW, Wang CH, Lin YC, Wu PL, Huang SH, Chang CC, Hu X, Gnatt A, Chang WH
Sample:Bovine RNA polymerase II elongation complex
Method:Single particle reconstruction (19 angstroms resolution)
Sample
Sample name: Bovine RNA polymerase II elongation complex
Theoretical molecular weight of the sample: 0.52
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1proteinRNA polymerase II elongation complex0.52monomerfalseBos taurus
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
81 mg/mL50 mM Tris-Cl, 200 mM KOAc, 5mM MgCl2, 5 mM DDT200 mesh quantifoil grid with thin carbon layer
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE80% KHOMEMADE PLUNGERBlot before plunging ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
JEOL 2100F200 kVSPOT SCANDARK FIELD mm nm nmFIELD EMISSION GUN mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
GATAN LIQUID NITROGEN°° eV K K K mrad20 e/Å222-FEB-2012
Processing
Software:Spider, EMAN2
CTF correction:Each particle
Number of particles:24000
Number of class averages:128
Imposed symmetry:C1
Resolution by author:19 Å
Resolution method:FSC 0.143
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
150 μm/pixel16ZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails