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Title:CryoEM icosahedral reconstruction of AHSV7
Authors:Manole V, Laurinmaki P, Wyngaardt WV, Potgieter CA, Wright IM, Venter GJ, Dijk AAV, Sewell BT, Butcher SJ
Sample:AHSV7 truncated VP2 filled
Method:Single particle reconstruction (11.4 angstroms resolution)
Red flagLatest update:2013-02-20
Sample
Sample name: AHSV7 truncated VP2 filled
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1virusAfrican horse sickness virus 7African horse sickness virus 7
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
8.5 mg/mL2mM Tris-HCl
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE100% KFEI VITROBOT MARK III ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F20200 kVFLOOD BEAMBRIGHT FIELD2 mm800 nm5000 nm8300083000FIELD EMISSION GUNGENERIC CCD mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
GATAN LIQUID NITROGEN°° eV K K K mrad18 e/Å201-FEB-2010
Processing
Protocol:polar Fourier transform
Software:AUTO3DEM
CTF correction:each micrograph
Number of particles:2798
Imposed symmetry:I
Resolution by author:11.4 Å
Resolution method:FSC 0.5
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
507 μm/pixel
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails