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Title:The Human Nuclear Pore Complex as Revealed by Cryo-Electron Tomography
Authors:Maimon T, Elad N, Dahan I, Medalia O
Sample:Asymmetric unit of the human nuclear pore complex
Method:Subtomogram averaging (66 angstroms resolution)
Red flagLatest update:2013-09-25
Sample
Sample name: Asymmetric unit of the human nuclear pore complex
Oligomeric state: octamer (actual refined structure represents an asymmetric unit of the NPC)
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1cellular-componentnuclear pore complexoctamerfalseHomo sapiens
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
mg/mLDulbecco phosphate buffer saline200 mesh gold grids, silicon coated
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KOTHERBlot for 4 seconds before plunging ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI POLARA 300300 kVFLOOD BEAMBRIGHT FIELD mm1200 nm1200 nm18000FIELD EMISSION GUN mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
nitrogen cooledGATAN LIQUID NITROGEN-60°60°GIF2002 eV90 K K K mrad e/Å201-JAN-2010
Processing
Protocol:missing wedge weighted correlation averaging
Software:TOM_toolbox_software_package
Number of subtomogram averaged:1696
Imposed symmetry:C1
Resolution by author:66 Å
Resolution method:FSC 0.5
Processing details:Manual selection.
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails