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Title:Structure of the Fab-Labeled 'Breathing' State of Native Poliovirus
Authors:Lin J, Lee L, Roivainen M, Filman DJ, Hogle JM, Belnap DM
Sample:Poliovirus 160S particle at 37 degrees C, Fab complex
Method:Single particle reconstruction (25 angstroms resolution)
Sample
Sample name: Poliovirus 160S particle at 37 degrees C, Fab complex
Oligomeric state: 160S particle icosahedral with Fab
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1virusHuman poliovirus 1 MahoneyHuman poliovirus 1 Mahoney
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.5 mg/mL20 mM Tris, 2 mM CaCl2
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KFEI VITROBOT MARK III ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F30300 kVFLOOD BEAMBRIGHT FIELD2 mm2395 nm3037 nm3900039000FIELD EMISSION GUNKODAK SO-163 FILM mmBsoft

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
GATAN LIQUID NITROGEN eV K K K mrad10 e/Å201-OCT-2007
Processing
Protocol:Fourier Bessel
Software:EM3DR2
CTF correction:CTF and decay correct
Number of particles:137
Imposed symmetry:I
Resolution by author:25 Å
Resolution method:FSC 0.5
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
77 μm/pixel8NIKON SUPER COOLSCAN 9000
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails