top_cornerHomeEMD-5342  Contact EMDataBank 
Image unavailable
Title:DNA-free p53 Tetramer Reconstruction
Authors:Pham N, Lucumi A, Cheung N, Viadiu H
Sample:p53
Method:Single particle reconstruction
Sample
Sample name: p53
Oligomeric state: tetramer
Experimental molecular weight of the sample: 0.2
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1proteinp53TetramertrueHomo sapiens
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
71 mg/mL150mM NaCl1% uranyl formate
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
NONE% KNONE ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI SPHERA200 kVSPOT SCANBRIGHT FIELD mm nm nmLAB6 mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
EucentricPHILIPS ROTATION HOLDER°° eV K K K mrad e/Å2
Processing
Number of particles:8550
Number of class averages:50
Imposed symmetry:C1
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
50 μm/pixel
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails