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Title:cryo-electron microscopy of microtubule-bound human kinesin-5 motor domain in the AMPPNP state (gold cluster in the N-terminus A9C).
Authors:GOULET A, MAJOR J, JUN Y, GROSS SP, ROSENFELD SR, MOORES CA
Sample:13-protofilament microtubule-bound human kinesin-5 motor domain with AMPPNP bound. A gold cluster is attached to the N-terminus (A9C).
Method:Single particle reconstruction (25 angstroms resolution)
Red flagLatest update:2014-02-12
Summary
Status: Released
Deposition date: 2013-12-30
Deposition site: PDBe
Processing site: PDBe
Header release date: 2014-01-15
Map release date: 2014-02-05
Primary citation: Comprehensive structural model of the mechanochemical cycle of a mitotic motor highlights molecular adaptations in the kinesin family.
Goulet A, Major J, Jun Y, Gross SP, Rosenfeld SS, Moores CA
PROC.NAT.ACAD.SCI.USA (2014) 111, pp. 1837-1842 [PubMed 24449904] [DOI]
Sample: 13-protofilament microtubule-bound human kinesin-5 motor domain with AMPPNP bound. A gold cluster is attached to the N-terminus (A9C).
Resolution: 25 Å (determined by FSC 0.5, semi-independent)