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Title:Injectisome in the tomogram of Salmonella mini-cell
Authors:Kawamoto A, Morimoto VY, Miyata T, Minamino T, Hughes TK, Kato T, Namba K
Sample:Injectisome from Salmonella typhimurium mini-cell in situ
Method:Subtomogram averaging (44 angstroms resolution)
Sample
Sample name: Injectisome from Salmonella typhimurium mini-cell in situ
Theoretical molecular weight of the sample: 3.5
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1cellular-componenttype III secretion system3.5falseSalmonella enterica subsp. enterica serovar Typhimurium
Experiment
Specimen state: Cell
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
mg/mLM9 medium (17.1g Na2HPO4-12H2O, 3g KH2PO4, 0.5g NaCl, 1g NH4Cl, 0.2%glycerol, 1% typtone, 1mM MgSO4 per litre)Quantifoil molybdenum 200 mesh R0.6/1.0 grid with thin carbon support
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE100% KFEI VITROBOT MARK II ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TITAN KRIOS300 kVFLOOD BEAMBRIGHT FIELD2.7 mm4000 nm7000 nm2900049030FIELD EMISSION GUNFEI FALCON I (4k x 4k) mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
FEI TITAN KRIOS AUTOGRID HOLDER-70°70° eV K80 K K mrad200 e/Å217-DEC-2012
Processing
Protocol:subtomogram averaging
Software:EMAN
Number of subtomogram averaged:75
Imposed symmetry:C24
Resolution by author:44 Å
Resolution method:FSC 0.5, semi-independent
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails