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Title:The electron miscroscopy reconstruction of the tail of lactococcal phage p2
Authors:Bebeacua C, Tremblay D, Farenc C, Chapot MP, Sadovskaya I, van Heel M, Veesler D, Moineau S, Cambillau C
Sample:Helical tail of Lactococcal phage p2
Method:Single particle reconstruction (22 angstroms resolution)
Red flagLatest update:2013-10-02
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Sample
Sample name: Helical tail of Lactococcal phage p2
Oligomeric state: Hexameric
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1virusLactococcus lactis phage p2Lactococcus lactis phage p2The tail fragmens were selected from a sample containing entire Lactococcal phages p2.
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.5 mg/mL50 mM Tris-HCl, 100 mM NaCl, 8 mM MgSO4Grids with adsorbed protein floated on 2% w/v uranyl acetate for 10 seconds.300 mesh gold grid with thin carbon support, glow discharged for 30 seconds.
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
NONE% KNONE ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM200T200 kVSPOT SCANBRIGHT FIELD2.2 mm1 nm2 nm38000FIELD EMISSION GUNCCD 4kx4k TVIPS mmObjective lens astigmatism was corrected at 100,000 times magnification.

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
SIDE ENTRY, EUCENTRIC°° eV K K K mrad10 e/Å201-JUL-2009
Processing
Protocol:Single particle, projection matching
Software:IMAGIC
CTF correction:Images
Number of particles:2171
Imposed symmetry:C6
Resolution by author:22 Å
Resolution method:FSC at 1/2-bit cut-off
Processing details:Helical reconstruction imposing C6 symmetry of the tail of lactococcal phage p2.
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
10004.64 μm/pixel
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails