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Title:The electron cryo-miscroscopy reconstruction of the connector of lactococcal phage p2
Authors:Bebeacua C, Tremblay D, Farenc C, Chapot MP, Sadovskaya I, van Heel M, Veesler D, Moineau S, Cambillau C
Sample:Connector of Lactococcal phage p2
Method:Single particle reconstruction (21 angstroms resolution)
Red flagLatest update:2013-10-02
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Sample
Sample name: Connector of Lactococcal phage p2
Oligomeric state: Dodecamer
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1virusLactococcus lactis phage p2Lactococcus lactis phage p2The connector was selected from a sample containing entire Lactococcal phages p2.
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.5 mg/mL50 mM Tris-HCl, 100 mM NaCl, 8 mM MgSO4Quantifoil grids
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
NONE% KNONE ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM200T200 kVSPOT SCANBRIGHT FIELD2.2 mm1 nm2 nm38000FIELD EMISSION GUNCCD 4kx4k TVIPS mmObjective lens astigmatism was corrected at 100,000 times magnification.

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
SIDE ENTRY, EUCENTRIC°° eV K K K mrad10 e/Å201-JUL-2009
Processing
Protocol:Single particle, projection matching
Software:IMAGIC
CTF correction:Images
Number of particles:1500
Imposed symmetry:C12
Resolution by author:21 Å
Resolution method:FSC at 1/2-bit cut-off
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
10004.64 μm/pixel
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails