top_cornerHomeEMD-2462  Contact EMDataBank 
Image unavailable
Title:Structure and Host Adhesion Mechanism of Virulent Lactococcal Phage p2
Authors:Bebeacua C, Tremblay D, Farenc C, Chapot MP, Sadovskaya I, van Heel M, Veesler D, Moineau S, Cambillau C
Sample:Capsid of Lactococcal phage p2
Method:Single particle reconstruction (13 angstroms resolution)
Red flagLatest update:2013-10-02
tab_top_blue.gif
bullet_white.gif  Summary
tab_bottom_blue.gif
pixel.gif
tab_top_white.gif
bullet.png Experimental details
tab_bottom_white.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Visualization
tab_bottom_blue.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Map information
tab_bottom_blue.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Downloads
tab_bottom_blue.gif
pixel.gif
Sample
Sample name: Capsid of Lactococcal phage p2
Oligomeric state: 60-mer
Theoretical molecular weight of the sample: 3
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1virusLactococcus lactis phage p2Lactococcus lactis phage p2The capsid was selected from a sample containing entire Lactococcal phages p2.
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.5 mg/mL50 mM Tris-HCl, 100 mM NaCl, 8 mM MgSO4Quantifoil grids
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE100% KFEI VITROBOT MARK IBlot for 2 seconds before plunging ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM200T200 kVSPOT SCANBRIGHT FIELD2.2 mm1 nm2 nm50000FIELD EMISSION GUNCCD 4kx4k TVIPS mmObjective lens astigmatism was corrected at 100,000 times magnification.

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
GATAN LIQUID NITROGEN°° eV K K K mrad10 e/Å201-JUL-2009
Processing
Protocol:Single particle, projection matching
Software:IMAGIC
CTF correction:Images
Number of particles:3329
Imposed symmetry:C1
Resolution by author:13 Å
Resolution method:FSC at 1/2-bit cut-off
Processing details:Reconstruction imposing Icosahedral reconstruction.
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
2003.53 μm/pixel
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
1OHG rigid bodyChimeraREAL60 copies of the hexamer of HK97 MCP were manually fitted and automatically refined with Chimera