top_cornerHomeEMD-2458  Contact EMDataBank 
Image unavailable
Title:30S Ribosome Subunit Assembly Intermediates, Intermediate Group1b
Authors:Mulder AM, Yoshioka C, Beck A, Bunner A, Milligan RA, Potter CS, Carragher B, Williamson JR
Sample:30S Reconstitution Reaction
Method:Single particle reconstruction (59.04 angstroms resolution)
Sample
Sample name: 30S Reconstitution Reaction
Theoretical molecular weight of the sample: 0.789
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1ribosome-prokaryote30S Ribosome SubunitfalseEscherichia coli
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.5 mg/mL25 mM Tris-HCl, 330 mM KCl, 20 mM MgCl2, 2 mM DTT
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
NONE% KNONE ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F20120 kVSPOT SCANBRIGHT FIELD mm nm nmFIELD EMISSION GUN mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Single tiltOTHER°° eV K K K mrad e/Å207-JAN-2009
Processing
Protocol:Random conical tilt
Software:Appion, Spider, Eman
CTF correction:Ace2
Number of particles:1827
Imposed symmetry:C1
Resolution by author:59.04 Å
Resolution method:FSC at 0.5
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
6635 μm/pixel
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails