EMD-2454 -- 30S Ribosome Reconstitution Reaction -- Mulder AM, Yoshioka C, Beck A, Bunner A, Milligan RA, Potter CS, Carragher B, Williamson JR -- EMDB

EMD-2454

Title:	30S Ribosome Subunit Assembly Intermediates, Intermediate Group 4b
Authors:	Mulder AM, Yoshioka C, Beck A, Bunner A, Milligan RA, Potter CS, Carragher B, Williamson JR
Sample:	30S Ribosome Reconstitution Reaction
Method:	Single particle reconstruction (40.87 angstroms resolution)

Sample

30S Ribosome Reconstitution Reaction

ribosome-prokaryote

30S Ribosome Subunit

false

Experiment

Particle

pH	Specimen conc.	Details	Staining	Specimen support details
7.5	mg/mL	25 mM Tris-HCl, 330 mM KCl, 20 mM MgCl2, 2 mM DTT	Aliquot from time point was adsorbed to grid and grid was immediately stained by adding a droplet of 2% UA	200 mesh copper grid with thin carbon support, glow discharged