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Title:The structure and super-organization of acetylcholine receptor-rapsyn complexes; class B
Authors:Zuber B, Unwin N
Sample:Postsynaptic membrane isolated from Torpedo marmorata electric organ
Method:Subtomogram averaging (41 angstroms resolution)
Red flagLatest update:2013-07-03
Sample name: Postsynaptic membrane isolated from Torpedo marmorata electric organ
Oligomeric state: acetylcholine receptors bound to a pair of rapsyns
Theoretical molecular weight of the sample: 0.382
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1proteinnicotinic acetylcholine receptor0.2900.280falseTorpedo marmorataIPR002394
2proteinRapsyn0.0430.046falseTorpedo marmorataGO:0007268, GO:0005515, GO:0008270, GO:0033130, GO:0005856, GO:0030054, GO:0045202, GO:0045211IPR001237, IPR019568, IPR011990, IPR013026, IPR013083, IPR001841, IPR019734, IPR001440, IPR018293the number of copies is suggested by the volume of the rapsyn density
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.4 mg/mL400 mM NaCl, 20 mM phosphate buffer, leupeptin 0.3 mg/l, pepstatin 1 mg/lunstained300 mesh copper grid with lacy carbon support, glow discharge in amylamine atmosphere
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE90%78 KHOMEMADE PLUNGERblot from the carbon side ms
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI POLARA 300300 kVFLOOD BEAMBRIGHT FIELD2 mm3000 nm6000 nm6900080213FIELD EMISSION GUNGATAN ULTRASCAN 4000 (4k x 4k) mmobjective lens astigmatism was corrected at 69,000 times magnification

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
GATAN LIQUID NITROGEN-70°70°Gatan0-20 eV85 K80 K95 K mrad50 e/Å2dual tilt axis tomography25-JUL-2008
Protocol:Simultaneous Iterative Reconstruction Technique (SIRT)
Number of subtomogram averaged:3564
Number of class averages:5
Imposed symmetry:C1
Resolution by author:41 Å
Resolution method:FSC 0.5
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
14215 μm/pixel16dual axis tilt series
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
2BG9 rigid bodyChimeraREAL