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Title:Electron cryo-microscopy of phosphorylation-mimicking mutants of alphaB-crystallin: the hexamer
Authors:Peschek J, Braun N, Rohrberg J, Back K, Kriehuber T, Kastenmueller A, Weinkauf S, Buchner J
Sample:Hexameric phosphorylation-mimicking mutant of alphaB-crystallin
Method:Single particle reconstruction (17 angstroms resolution)
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Sample
Sample name: Hexameric phosphorylation-mimicking mutant of alphaB-crystallin
Oligomeric state: Hexameric
Theoretical molecular weight of the sample: 0.121
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1proteinHexameric phosphorylation-mimicking mutant of human alphaB-crystallinHexamertrueHomo sapiensP02511Hexameric phosphorylation-mimicking mutant of human alphaB-crystallin
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.40.2 mg/mLPBSCryo
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KNONE ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
OTHER120 kVSPOT SCANBRIGHT FIELD mm900 nm1800 nm5000049415LAB6 mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
GATAN LIQUID NITROGEN°° eV K K K mrad e/Å202-SEP-2010
Processing
Protocol:Projection matching
Software:Imagic
CTF correction:Each negative
Number of particles:5617
Imposed symmetry:C3
Resolution by author:17 Å
Resolution method:FSC at 0.5 cut-off
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
μm/pixelOTHER
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails