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Title:Structure and conformational variability of the Mycobacterium tuberculosis fatty acid synthase multienzyme complex
Authors:Ciccarelli L, Connell SR, Enderle M, Mills DJ, Vonck J, Grininger M
Sample:M. tuberculosis type-I fatty acid synthase
Method:Single particle reconstruction (17.5 angstroms resolution)
Red flagLatest update:2013-07-17
Sample
Sample name: M. tuberculosis type-I fatty acid synthase
Experimental molecular weight of the sample: 2.0
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1proteinType-I fatty acid synthase2.02.0HomohexamertrueMycobacterium tuberculosis
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
mg/mLQuantifoils copper grid
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KFEI VITROBOT MARK III ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI POLARA 300200 kVFLOOD BEAMBRIGHT FIELD mm1800 nm4500 nm59000FIELD EMISSION GUNKODAK SO-163 FILM mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
GATAN LIQUID NITROGEN°° eV K K K mrad e/Å216-DEC-2010
Processing
Software:EMAN2, SPARX
Number of particles:9136
Imposed symmetry:C1
Resolution by author:17.5 Å
Resolution method:FSC 0.5
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
μm/pixelZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
3zen rigid bodyREAL