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Title:cryo-EM structure of the NavCt voltage-gated sodium channel
Authors:Tsai C-J, Tani K, Irie K, Hiroaki Y, Shimomura T, McMillan DG, Cook GM, Schertler G, Fujiyoshi Y, Li X-D
Sample:voltage-gated sodium channel
Method:2D crystallography (9.0 angstroms resolution)
Red flagLatest update:2013-11-06
Sample
Sample name: voltage-gated sodium channel
Oligomeric state: Two tetramers of voltage-gated sodium channel
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1proteinvoltage-gated sodium channeltetramertrueCaldalkalibacillus thermarumF5L478GO:0005216IPR005821
Experiment
Specimen state: 2D array
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
9.04.0 mg/mL50mM glycine-NaOH pH9.0, 200mM NaCl, 4mM MgCl2, 5% glycerol, 5% methyl-2,4-pentanediol, 1.5mM NaN3molybdenum EM grid
Crystal growth details:Crystals grown by dialysis
Plane group:P 4
Unit cell:
abGamma
115.0 Å115.0 Å90.0°
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
NITROGEN% KLEICA KF80 ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
JEOL KYOTO-3000SFF300 kVFLOOD BEAMBRIGHT FIELD1.6 mm910 nm3820 nm4000039500FIELD EMISSION GUNKODAK SO-163 FILM mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Helium cooled, top entryJEOL60° eV4 K4 K K mrad20 e/Å205-NOV-2010
Processing
Software:MRC
CTF correction:Each micrographs
Resolution by author:9.0 Å
Resolution method:Phase residuals
Processing details:Images were processed using MRC suite.
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
777 μm/pixel14ZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
3RVY CNSREAL