EMD-2321

Single-particle
25.0 Å
EMD-2321
3D View Gallery
Deposition: 26/02/2013
Map released: 17/04/2013
Last modified: 24/04/2013
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EMD-2321
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3D volume (map.gz) Experimental metadata (xml)
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EMICSS annotations (xml)

The bacterial DnaC helicase loader is a DnaB ring breaker

EMD-2321

Single-particle
25.0 Å
EMD-2321
3D View Gallery
Deposition: 26/02/2013
Map released: 17/04/2013
Last modified: 24/04/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 8.5
Details: 20 mM Tris-HCl pH 8.5, 200 mM NaCl, 5 % glycerol, 5 mM MgCl2, 1 mM beta-mercaptoethanol, and 1 mM ADP-BeF3
Staining
Type: NEGATIVE
Details: Grids with adsorbed protein were floated on 2% w/v uranyl formate for 45 seconds
Grid
Details: 400 mesh copper grid with thin carbon support, glow discharged for 20 seconds
Vitrification
Cryogen name: NONE
Instrument: OTHER
Microscopy [1]
Microscope: FEI TECNAI 12
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: LAB6
Acceleration voltage: 120 kV
Nominal CS: 6.3 mm
Nominal defocus: 0.7 µm - 1.2 µm
Nominal magnification: 49000.0
Specimen holder model: SIDE ENTRY, EUCENTRIC
Temperature
Average: 297 K
Image Recording [1]
Detector category: CCD
Detector model: GENERIC TVIPS (4k x 4k)
Average electron dose per image: 25 e/Å2
Image processing [1]
Details: The particles were selected using DoG picker as available in APPION. The contrast transfer function of the microscope for each micrograph was estimated using CTFFIND3 and phase-flipped using SPIDER. DnaBC particles were subjected to a multi-model refinement as implemented in SPARX using the 3D averages obtained from the RCT reconstructions as initial references.
Final reconstruction
Resolution: 25.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 17942
Algorithm: OTHER
Details:
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
EMAN2, SPARX - -
CTF correction
Details:Each micrograph
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Negative staining reconstruction of E. coli DnaB/DnaC complex
Details: ::::EMDATABANK.org::::EMD-2321::::
⬡ Geometry
X Y Z
Dimensions 80 80 80
Origin 0 0 0
Spacing 80 80 80
Voxel size 4.36 Å 4.36 Å 4.36 Å
Contour list
Primary Level Source
True 3.0 AUTHOR