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Title:Negative stain EM structure of TcdA1 in pore state
Authors:Gatsogiannis C, Lang A E, Meusch D, Pfaumann V, Hofnagel O, Benz R, Aktories K, Raunser S
Sample:TcdA1
Method:Single particle reconstruction (26.5 angstroms resolution)
Red flagLatest update:2013-04-03
Sample
Sample name: TcdA1
Oligomeric state: Pentamer
Experimental molecular weight of the sample: 1.41
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1proteinTcdA10.2830.283truePhotorhabdus luminescensQ9RN43
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
110.02 mg/mL50 mM CAPS, mM NaCl, 0.05% Tween-20, 5% glycerolGrids with adsorbed Protein floated on 0.07% Uranyl Formate for 45 sec.glow discharged 400 mesh copper grids with thin carbon support
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
NONE% KNONE ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
JEOL 1400120 kVFLOOD BEAMBRIGHT FIELD3.4 mm1.2 nm2 nm5000067210LAB6TVIPS TEMCAM-F416 (4k x 4k) mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
JEOL°° eV K K K mrad e/Å228-SEP-2011
Processing
Protocol:Projection Matching
Software:Sparx
Number of particles:1936
Imposed symmetry:C5
Resolution by author:26.5 Å
Resolution method:FSC 0.5
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
80 μm/pixel
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails