EMD-2299

Single-particle
24.6 Å
EMD-2299
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Deposition: 25/01/2013
Map released: 13/02/2013
Last modified: 03/04/2013
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EMD-2299
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Negative stain EM structure of the PTC3 holotoxin complex (TcdA1, TcdB2, TccC3) in prepore state

EMD-2299

Single-particle
24.6 Å
EMD-2299
3D View Gallery
Deposition: 25/01/2013
Map released: 13/02/2013
Last modified: 03/04/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 5.0
Details: 50 mM MES, 100 mM NaCl, 0.05% Tween-20, 5% glycerol
Staining
Type: NEGATIVE
Details: Grids with adsorbed Protein floated on 0.07% Uranyl Formate for 45 sec.
Grid
Details: Glow discharged 400 mesh copper grids with thin carbon support
Vitrification
Cryogen name: NONE
Instrument: OTHER
Microscopy [1]
Microscope: JEOL 1400
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: LAB6
Acceleration voltage: 120 kV
Nominal CS: 3.4 mm
Nominal defocus: 0.0012 µm - 0.002 µm
Nominal magnification: 50000.0
Calibrated magnification: 67210.0
Specimen holder model: JEOL
Image Recording [1]
Detector category: CCD
Detector model: TVIPS TEMCAM-F416 (4k x 4k)
Number of real images: 84
Image processing [1]
Details: Partial-symmetry projection matching approach (after each refinement round, C5 symmetry was applied for the TcdA1 component)
Final reconstruction
Resolution: 24.6 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 3022
Algorithm: OTHER
Details:
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
Sparx - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of PTC3 holotoxin complex in prepore state
Details: ::::EMDATABANK.org::::EMD-2299::::
⬡ Geometry
X Y Z
Dimensions 112 112 112
Origin 0 0 0
Spacing 112 112 112
Voxel size 4.64 Å 4.64 Å 4.64 Å
Contour list
Primary Level Source
True 0.021 AUTHOR