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Title:Structure of TcdA1 pore (TcdA1 reconstituted in liposomes)
Authors:Gatsogiannis C, Lang A E, Meusch D, Pfaumann V, Hofnagel O, Benz R, Aktories K, Raunser S
Method:Single particle reconstruction (19.9 angstroms resolution)
Red flagLatest update:2013-04-03
Sample name: TcdA1
Oligomeric state: Pentamer
Experimental molecular weight of the sample: 1.41
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1proteinTcdA10.2830.283truePhotorhabdus luminescensQ9RN43Protein reconstituted in liposomes
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
11 mg/mL50 mM CAPS pH 11, 100 mM NaCl, 5 % glycerolC-Flat 1.2/1.3-4C copper 400 mesh, with additional thin carbon support
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE90%100 KGATAN CRYOPLUNGE 3Blot for 1 sec before plunging ms
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
JEOL 3200FSC200 kVFLOOD BEAMBRIGHT FIELD4.1 mm2.9 nm5 nm4000085460FIELD EMISSION GUNTVIPS TEMCAM-F816 (8k x 8k) mmObjective lens astigmatism was corrected at 120,000 times magnification

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
JEOL 3200FSC CRYOHOLDER°°in-column Omega filter0-15 eV K K K mrad20 e/Å218-MAR-2012
Protocol:Projection matching
CTF correction:Each Particle
Number of particles:5549
Imposed symmetry:C5
Resolution by author:19.9 Å
Resolution method:FSC 0.5
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
145 μm/pixel
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails