EMD-2293

Single-particle
10.0 Å
EMD-2293
3D View Gallery
Deposition: 21/01/2013
Map released: 13/03/2013
Last modified: 24/07/2013
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EMD-2293
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Second 3D model of the MjHSP16.5 mutant R107G at room temperature by CryoEM.

EMD-2293

Single-particle
10.0 Å
EMD-2293
3D View Gallery
Deposition: 21/01/2013
Map released: 13/03/2013
Last modified: 24/07/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.8 mg/mL
Buffer
pH: 7.4
Details: 10mM HEPES, 100mM NaCl.
Grid
Details: GIG holey grids (LifeTrust, China) were treated with a glow discharge machine (Master Plasmer)
Vitrification
Cryogen name: ETHANE
Chamber temperature: 93 K
Instrument: FEI VITROBOT MARK IV
Method: The samples were blotted for 2 s with blot force 2 at 100% humidity.
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal defocus: 2.0 µm - 3.0 µm
Nominal magnification: 75000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Specimen holder details: Liquid nitrogen cooled
Temperature
Average: 85 K
Image Recording [1]
Detector category: CCD
Detector model: GATAN ULTRASCAN 4000 (4k x 4k)
Number of real images: 546
Average electron dose per image: 20 e/Å2
Bits per pixel: 32.0
Details: Electron micrographs were collected manually.
Image processing [1]
Details: The particles were selected using an automatic selection program Gautomatch developed in Fei Sun lab (to be published). Octahedron symmetry were imposed during 3D reconstructing.
Final reconstruction
Resolution: 10.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 5420
Algorithm: OTHER
Details: The final reconstructed density map was further sharpened by application of an amplitude correction algorithm in the program BFACTOR
⌯ Applied Symmetry
Point group: O
Software [1]
Name Version Details
EMAN1 - -
Final 2D classification
Number of classes: 193
CTF correction
Details:Each image
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Second model for reconstruction of mutant MjHSP16.5 R107G at room temperature
Details: ::::EMDATABANK.org::::EMD-2293::::
⬡ Geometry
X Y Z
Dimensions 200 200 200
Origin -100 -100 -100
Spacing 200 200 200
Voxel size 1.196 Å 1.196 Å 1.196 Å
Contour list
Primary Level Source
True 2.53 AUTHOR