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Title:cryoEM structure of hepatitis B virus core assembled from full-length core protein
Authors:Yu X, Jin L, Jih J, Shih C, Zhou ZH
Sample:Hepatitis B virus core assembled from full-length core protein
Method:Single particle reconstruction (3.5 angstroms resolution)
Sample
Sample name: Hepatitis B virus core assembled from full-length core protein
Oligomeric state: Icosahedral particle
Experimental molecular weight of the sample: 5
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1virusHepatitis B virus55Hepatitis B virus
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
mg/mL
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
NITROGEN100%120 KFEI VITROBOT MARK II ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TITAN KRIOS300 kVFLOOD BEAMBRIGHT FIELD2.75 mm nm nm75000FIELD EMISSION GUN mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
GATAN LIQUID NITROGEN°° eV K K K mrad25 e/Å201-JAN-2009
Processing
Protocol:Cross-common lines
Software:IMIRS
CTF correction:Each particle
Number of particles:8093
Imposed symmetry:I
Resolution by author:3.5 Å
Resolution method:FSC 0.143
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
μm/pixelNIKON SUPER COOLSCAN 9000
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails