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Title:Electron cryo-microscopy of a head-tailed virus infecting extremely halophilic archaea
Authors:Pietila MK, Laurinmaki P, Russell DA, Ko CC, Jacobs-Sera D, Butcher SJ, Bamford DH, Hendrix RW
Sample:archaeal virus HSTV-2
Method:Single particle reconstruction (9.8 angstroms resolution)
Red flagLatest update:2013-03-06
Sample
Sample name: archaeal virus HSTV-2
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1virusHSTV-2HSTV-2
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.2 mg/mL20mM Tris-HClQuantifoil holey carbon film on 300 mesh copper grid.
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KHOMEMADE PLUNGER ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F20200 kVFLOOD BEAMBRIGHT FIELD2.0 mm700 nm2900 nm6200062000FIELD EMISSION GUNKODAK SO-163 FILM mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
GATAN LIQUID NITROGEN°° eV K K K mrad e/Å203-DEC-2009
Processing
Software:AUTO3DEM
Number of particles:4930
Imposed symmetry:I
Resolution by author:9.8 Å
Resolution method:FSC 0.5
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
7 μm/pixel12ZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails