top_cornerHomeEMD-2234  Contact EMDataBank 
Image unavailable
Title:Electron cryo-microscopy of a head-tailed virus infecting extremely halophilic archaea
Authors:Pietila MK, Laurinmaki P, Russell DA, Ko CC, Jacobs-Sera D, Butcher SJ, Bamford DH, Hendrix RW
Sample:archaeal virus HVTV-1
Method:Single particle reconstruction (10.5 angstroms resolution)
Red flagLatest update:2013-03-06
Sample
Sample name: archaeal virus HVTV-1
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1virusHVTV-1HVTV-1
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.2 mg/mL20mM Tris-HClQuantifoil holey carbon film on 300 mesh copper grid.
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KHOMEMADE PLUNGER ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F20200 kVFLOOD BEAMBRIGHT FIELD2.0 mm400 nm2900 nm6200062000FIELD EMISSION GUNKODAK SO-163 FILM mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
GATAN LIQUID NITROGEN°° eV K K K mrad e/Å208-OCT-2009
Processing
Software:AUTO3DEM
Number of particles:3530
Imposed symmetry:I
Resolution by author:10.5 Å
Resolution method:FSC 0.5
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
7 μm/pixel12ZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails