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Title:Staphylothermus marinus 50S ribosomal subunit
Authors:Armache J-P, Anger AM, Marquez V, Frankenberg S, Froehlich T, Villa E, Berninghausen O, Thomm M, Arnold GJ, Beckmann R, Wilson DN
Sample:Staphylothermus marinus 50S ribosomal subunit
Method:Single particle reconstruction (24 angstroms resolution)
Sample
Sample name: Staphylothermus marinus 50S ribosomal subunit
Oligomeric state: One ribosomal subunit
Experimental molecular weight of the sample: 1.5
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1ribosome-prokaryoteStaphylothermus marinus 50S ribosomal subunit1.51.5falseStaphylothermus marinus
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.5 mg/mL20 mM Hepes pH 7.5, 10 mM Mg(OAc)2, 30 mM NH4OAc, 4 mM beta-Mercaptoethanol
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE100% KFEI VITROBOT MARK IIIBlot for 10 seconds before plunging, use 2 layers of filter paper msVitrification instrument: Vitrobot
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI 12120 kVFLOOD BEAMBRIGHT FIELD2.0 mm nm nm9000090000LAB6FEI EAGLE (4k x 4k) mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
GATAN LIQUID NITROGEN°° eV K K K mrad20 e/Å206-SEP-2008
Processing
Protocol:Single Particle
Software:SPIDER
CTF correction:Wiener filter on 3D volumes
Number of particles:11142
Imposed symmetry:C1
Resolution by author:24 Å
Resolution method:FSC 0.5
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
99 μm/pixelData collected on CCDOTHER
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
3CC2 rigid bodyChimeraREALProtocol: Rigid body. Rigid-body fit into the density using UCSF Chimera built-in "Fit in Map"