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Title:Electron cryo-microscopy of the intact (DNA-filled) head of the yersiniophage phiR1-37
Authors:Skurnik M, Hyytiainen H, Happonen LJ, Kiljunen S, Datta N, Mattinen L, Williamson K, Kristo P, Szeliga M, Kalin-Manttari L, Ahola-Iivarinen E, Kalkkinen N, Butcher SJ
Sample:Electron cryo-microscopy of the intact (DNA-filled) head of the yersiniophage phiR1-37
Method:Single particle reconstruction (23.8 angstroms resolution)
Red flagLatest update:2012-10-24
Sample
Sample name: Electron cryo-microscopy of the intact (DNA-filled) head of the yersiniophage phiR1-37
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1virusYersinia phage phiR1-37Yersinia phage phiR1-37
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
mg/mL50 mM Tris pH 7.8, 10 mM MgSO4
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KHOMEMADE PLUNGER ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F20200 kVFLOOD BEAMBRIGHT FIELD2.0 mm500 nm5760 nm62000FIELD EMISSION GUNKODAK SO-163 FILM mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Side entry liquid nitrogen-cooled cryo specimen holderGATAN LIQUID NITROGEN°° eV K K K mrad15 e/Å2Low dose conditions07-SEP-2009
Processing
Software:AUTO3DEM
CTF correction:Each micrograph
Number of particles:1028
Imposed symmetry:I
Resolution by author:23.8 Å
Resolution method:FSC 0.5
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
297 μm/pixelZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails