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Title:Negative structure of open and closed conformation of crm1
Authors:Monecke T, Haselbach D, Neumann P, Thomson E, Hurt E, Stark H, Dickmanns A, Ficner R
Sample:crm1 open conformation
Method:Single particle reconstruction (18 angstroms resolution)
Sample
Sample name: crm1 open conformation
Oligomeric state: monomer
Theoretical molecular weight of the sample: 0.12
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1proteincrm10.12monomertrueChaetomium thermophilum
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.5 mg/mL150 mM NaCl, 20 mM HEPES/NaOH pH 7.5, 2 mM MgCl2, 4 mM DTTGrids with adsorbed protein floated on 1% w/v uranyl formate for 60 seconds200 mesh copper grid with thin carbon support, freshly floated on protein solution
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
NONE% KNONE ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM200FEG160 kVSPOT SCANBRIGHT FIELD mm nm nm155000FIELD EMISSION GUNGENERIC TVIPS (4k x 4k) mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
OTHER°° eV298 K K K mrad e/Å221-MAR-2012
Processing
Protocol:Cross-common-lines
Software:imagic
CTF correction:each particle
Number of particles:21000
Resolution by author:18 Å
Resolution method:FSC 0.5
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
300 μm/pixel
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails