top_cornerHomeEMD-2070  Contact EMDataBank 
Image unavailable
Title:Octameric structure of IMPDH from Pseudomonas aeruginosa in presence of Mg-ATP
Authors:Labesse G, Vaupre L, alard-Arnaud I, Alexandre T, Lai Kee Him J, Raynal B, Bron P, Munier-Lehmann H
Sample:IMPDH from Pseudomonas aeruginosa in presence of Mg-ATP filtered at 16A resolution
Method:Single particle reconstruction (16 angstroms resolution)
Red flagLatest update:2014-03-26
Sample
Sample name: IMPDH from Pseudomonas aeruginosa in presence of Mg-ATP filtered at 16A resolution
Oligomeric state: octameric
Theoretical molecular weight of the sample: 0.43
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1proteinInosine-5'-monophosphate dehydrogenase0.43octamertruePseudomonas aeruginosa
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
81 mg/mL20 mM potassium phosphate pH 8 and 0.1 M KClQuantifoil R2.2, glow discharged
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE98%102.15 KGATAN CRYOPLUNGE 3Blot for 1 second ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
JEOL 2200FS200 kVFLOOD BEAMBRIGHT FIELD2.0 mm1400 nm2500 nm100000130137FIELD EMISSION GUNGENERIC GATAN (4k x 4k) mmObjective lens astigmatism was corrected at 200,000 times magnification

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
626 Gatan cryo-HolderGATAN LIQUID NITROGEN°°JEOL0-20 eV95.15 K93.15 K96.15 K mrad18 e/Å2Image acquisition is done directly on the CCD camera. It explains why the nominal magnification is 100000 and calibrated magnification 130137.01-NOV-2011
Processing
Software:IMAGIC, FREALIGN
CTF correction:Each particle
Number of particles:22577
Imposed symmetry:D4
Resolution by author:16 Å
Resolution method:FSC 0.5
Processing details:The particles were selected using a semi-automatic selection program (Boxer).
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
80 μm/pixelImages directly recorded with CCD camera
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails