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Title:CryoEM 3D reconstruction of full Triatoma Virus
Authors:Neumann E, Agirre J, Goret G, Legoff M, Marti GA, Navaza J, Guerin DMA
Sample:Full Triatoma Virus
Method:Single particle reconstruction (15 angstroms resolution)
Red flagLatest update:2014-10-08
Sample
Sample name: Full Triatoma Virus
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1virusTriatoma virusTriatoma virus
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.4 mg/mLNMT (10 mM NaCl, 1 mM MgCl2, 50 mM Tris-HCl)400 mesh quantifoil grid
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KFEI VITROBOT msVitrification instrument: Vitrobot FEI
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
JEOL 2010F200 kVOTHERBRIGHT FIELD1.4 mm1200 nm4000 nm40000FIELD EMISSION GUNKODAK SO-163 FILM mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Side entry liquid nitrogen-cooled cryo specimen holderGATAN LIQUID NITROGEN°° eV K K K mrad e/Å2
Processing
Software:EM3DR2
Number of particles:1716
Imposed symmetry:I
Resolution by author:15 Å
Resolution method:FSC 0.5
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
257 μm/pixelZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails