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Title:Structure of the CS1 pilus of enterotoxigenic Escherichia coli reveals structural polymorphism
Authors:Galkin VE, Kolappan S, Ng D, Zong Z, Li J, Yu X, Egelman EH, Craig L
Sample:CS1 pilus
Method:Helical reconstruction (20 angstroms resolution)
Red flagLatest update:2013-03-20
Sample
Sample name: CS1 pilus
Oligomeric state: oligomer
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1cellular-componentCooA0.015OligomerfalseEscherichia coli
Experiment
Specimen state: Filament
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.40.2 mg/mL137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 2 mM KH2PO4, 10 mM EDTA2% uranyl acetateglow discharged continuous carbon coated grids
Helical parameters:
HandPhi incrementAzimuthal (Z) increment
RIGHT HANDED113.1°8.6 Å
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
NONE% KNONE ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI 1280 kVOTHERBRIGHT FIELD2 mm1000 nm2000 nm3000030000LAB6KODAK SO-163 FILM mmobjective lens astigmatism was corrected at 100,000 times magnification

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
EucentricSIDE ENTRY, EUCENTRIC°° eV K K K mrad10 e/Å2Low dose mode
Processing
Protocol:single particle reconstruction
Software:SPIDER, IHRSR
Resolution by author:20 Å
Resolution method:FSC 0.5
Processing details:Pilus segments were selected using the helixboxer program in SPIDER.
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
104.16 μm/pixelNIKON COOLSCAN
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
3S0V rigid bodyREALProtocol: Rigid body. A single subunit was manually docked using CHIMERA and the filament was built by applying the symmetry parameters for the reconstruction.