Contribute data to the DEN

If you are interested in contributing data, contact Wim Vranken.

Data requirements per protein (for peak analysis)

1. Amino acid sequence of the protein


2. Contact information (in case there are questions about the data)


3. Per dataset (as many as possible) for the protein:
1. Raw time domain data and original Bruker/Varian acquistion file(s)
2. Processing parameters per dimension. These are important for handling the data (note that the nmrPipe, azara,... processing script used for this spectrum is equally useful):
   1. Phase settings
   2. Negotiation of alternate points? (ie x y -x -y data acquistion)
   3. Echo-antiecho (Bruker) / Rance-Kay (Varian)?
   4. Varian acquisition order (if d3, d2, phase, phase2)?
   5. Should data be conjugated?


4. Sample information (pH, temperature, protein & buffer concentrations, volume)


5. Spectrometer information (manufacturer, model, field)


6. Your peak list for this spectrum (in any format)


7. If possible: software used for analysis (processing, peak picking,...)


8. If possible: probe information

Additional data per protein (for structure validation)

1. PDB file with structure coordinates (or PDB code if already submitted)


2. Resonance chemical shift assignments (or BMRB code if already submitted)


3. Restraints list(s) used for calculation (or PDB/BMRB code if already submitted)


4. If possible: information on dynamics (heteronuclear, T1, T2)