› 9xia

X-ray diffraction
1.9Å resolution

X-RAY ANALYSIS OF D-XYLOSE ISOMERASE AT 1.9 ANGSTROMS: NATIVE ENZYME IN COMPLEX WITH SUBSTRATE AND WITH A MECHANISM-DESIGNED INACTIVATOR

Released:
DOI: 10.2210/pdb9xia/pdb
PDB entry 9xia coloured by chain, front view.
PDB entry 9xia coloured by chain, side view.
PDB entry 9xia coloured by chain, top view.
Source organism: Streptomyces rubiginosus
Primary publication:
X-ray analysis of D-xylose isomerase at 1.9 A: native enzyme in complex with substrate and with a mechanism-designed inactivator.
Carrell HL, Glusker JP, Burger V, Manfre F, Tritsch D, Biellmann JF
Proc Natl Acad Sci U S A 86 4440-4 (1989)
PMID: 2734296

Function and Biology Details

Reaction catalysed: 
Alpha-D-xylopyranose = alpha-D-xylufuranose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150204 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Xylose isomerase Chain: A
Molecule details ›
Chain: A
Length: 388 amino acids
Theoretical weight: 43.25 KDa
Source organism: Streptomyces rubiginosus
Expression system: Not provided
UniProt:
  • Canonical: P24300 (Residues: 1-388; Coverage: 100%)
Gene name: xylA
Sequence domains: Xylose isomerase-like TIM barrel
Structure domains: Divalent-metal-dependent TIM barrel enzymes

Ligands and Environments

2 bound ligands:
Ligand DFR 1 x DFR
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: I222
Unit cell:
a: 93.8Å b: 100Å c: 103Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.141 not available not available
Expression system: Not provided

Quick links

Citations

5 review citations

Mechanisms and free energies of enzymatic reactions.
Gao et al. (2006)
4 more

2 mentions without citation

From the similarity analysis of protein cavities to the functional classification of protein families using cavbase.
Kuhn et al. (2006)
1 more