› 8dfr

X-ray diffraction
1.7Å resolution

REFINED CRYSTAL STRUCTURES OF CHICKEN LIVER DIHYDROFOLATE REDUCTASE. 3 ANGSTROMS APO-ENZYME AND 1.7 ANGSTROMS NADPH HOLO-ENZYME COMPLEX

Released:
DOI: 10.2210/pdb8dfr/pdb
PDB entry 8dfr coloured by chain, front view.
PDB entry 8dfr coloured by chain, side view.
PDB entry 8dfr coloured by chain, top view.
Source organism: Gallus gallus
Entry authors: Matthews DA, Oatley SJ, Burridge J, Kaufman BT, Xuong N-H, Kraut J

Function and Biology Details

Reaction catalysed: 
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132451 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate reductase Chain: A
Molecule details ›
Chain: A
Length: 189 amino acids
Theoretical weight: 21.68 KDa
Source organism: Gallus gallus
Expression system: Not provided
UniProt:
  • Canonical: P00378 (Residues: 1-189; Coverage: 100%)
Gene name: DHFR
Sequence domains: Dihydrofolate reductase
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments


Cofactor: Ligand NDP 1 x NDP
1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 89.07Å b: 47.96Å c: 64.31Å
α: 90° β: 124.84° γ: 90°
R-values:
R R work R free
0.188 not available not available
Expression system: Not provided

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