PDBe Entry: 7r1r

RIBONUCLEOTIDE REDUCTASE E441Q MUTANT R1 PROTEIN FROM ESCHERICHIA COLI

Summary

Header

COMPLEX (OXIDOREDUCTASE/PEPTIDE)

Method

X-RAY DIFFRACTION

Experiment

Resolution: 3.1 Å, R-factor: 20.5%, Free R-factor: 23.1%, Spacegroup: H 3 2

Released

18/03/1998, deposition: 17/09/1997, last revision: 24/02/2009

Authors

Eriksson, M.

; Eklund, H.

Primary citation

A new mechanism-based radical intermediate in a mutant R1 protein affecting the catalytically essential Glu441 in Escherichia coli ribonucleotide reductase.
J.BIOL.CHEM.

vol:272, pag:31533-31541 (1997) [PubMed ID 9395490 ]

Keywords

RIBONUCLEOTIDE REDUCTASE

, DEOXYRIBONUCLEOTIDE SYNTHESIS

, RADICAL CHEMISTRY

, ALLOSTERIC REGULATION

, SPECIFICITY

, COMPLEX (OXIDOREDUCTASE/PEPTIDE)

1.17.4.1 ExPASy BRENDA

(A D B E C F P)

Organism

Escherichia coli 562

(A B C D E F P)

UniProt

Ribonucleoside-diphosphate reductase 1 subunit alpha (EC 1.17.4.1) (Ribonucleoside-diphosphate reductase 1 R1 subunit) (Ribonucleotide reductase 1) (B1 protein) P00452

(A B C)
Ribonucleoside-diphosphate reductase 1 subunit beta (EC 1.17.4.1) (Ribonucleotide reductase 1) (B2 protein) (R2 protein) P69924

(D E F P)

Solvent

A, B, C

Polymers

Id	Name	Type	UniProt	Residues	Observed
A, B, C	RIBONUCLEOTIDE REDUCTASE R1 PROTEIN	Protein	P00452 (RIR1_ECOLI)	761	96%
D, E, F, P	RIBONUCLEOTIDE REDUCTASE R2 PROTEIN	Protein	P69924 (RIR2_ECOLI)	20	90%