› 7cry

X-ray diffraction
1.85Å resolution

Structure of Chloride ion pumping rhodopsin (ClR) with NTQ motif 100 ps after light activation (6.49 mJ/mm2)

Released:
DOI: 10.2210/pdb7cry/pdb
PDB entry 7cry coloured by chain, front view.
PDB entry 7cry coloured by chain, side view.
PDB entry 7cry coloured by chain, top view.
Source organism: Nonlabens marinus S1-08
Primary publication:
Early-stage dynamics of chloride ion-pumping rhodopsin revealed by a femtosecond X-ray laser.
Yun JH, Li X, Yue J, Park JH, Jin Z, Li C, Hu H, Shi Y, Pandey S, Carbajo S, Boutet S, Hunter MS, Liang M, Sierra RG, Lane TJ, Zhou L, Weierstall U, Zatsepin NA, Ohki M, Tame JRH, Park SY, Spence JCH, Zhang W, Schmidt M, Lee W, Liu H
Proc Natl Acad Sci U S A 118 (2021)
PMID: 33753488

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197820 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chloride pumping rhodopsin Chain: A
Molecule details ›
Chain: A
Length: 264 amino acids
Theoretical weight: 29.4 KDa
Source organism: Nonlabens marinus S1-08
Expression system: Escherichia coli
UniProt:
  • Canonical: W8VZW3 (Residues: 2-265; Coverage: 97%)
Gene names: ClR, NMS_1267
Sequence domains: Bacteriorhodopsin-like protein

Ligands and Environments

3 bound ligands:
Ligand CL 2 x CL
Ligand RET 1 x RET
Ligand OLA 7 x OLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLAC LCLS BEAMLINE CXI
Spacegroup: C2
Unit cell:
a: 103.37Å b: 50.09Å c: 69.39Å
α: 90° β: 109.69° γ: 90°
R-values:
R R work R free
0.261 0.258 0.321
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Rhodopsins: An Excitingly Versatile Protein Species for Research, Development and Creative Engineering.
de Grip et al. (2022)
3 more