› 7b0h

X-ray diffraction
3.15Å resolution

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121094 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase Chains: E, F
Molecule details ›
Chains: E, F
Length: 773 amino acids
Theoretical weight: 89.88 KDa
Source organism: Thermococcus gorgonarius
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P56689 (Residues: 1-773; Coverage: 100%)
Gene names: pol, polA
Sequence domains:
DNA (5'-D(P*AP*AP*CP*GP*GP*CP*AP*AP*AP*TP*GP*CP*G)-3') Chains: A, C
Molecule details ›
Chains: A, C
Length: 13 nucleotides
Theoretical weight: 4.01 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*CP*GP*CP*AP*TP*T)-3') Chains: D, G
Molecule details ›
Chains: D, G
Length: 6 nucleotides
Theoretical weight: 1.78 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand MN 4 x MN
Ligand MG 2 x MG
Ligand TTP 2 x TTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 111.823Å b: 112.331Å c: 186.518Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.232 0.247
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 citation in other articles

Structural dynamics and determinants of 2-aminoadenine specificity in DNA polymerase DpoZ of vibriophage ϕVC8.
Czernecki et al. (2021)
2 more

1 mention without citation

Spatial arrangement of proteins in planar and curved membranes by PPM 3.0.
Lomize et al. (2022)