› 7asv

X-ray diffraction
1.55Å resolution

Crystal structure of tWHD2 of Rpc5 subunit of human RNA Polymerase III

Released:
DOI: 10.2210/pdb7asv/pdb
PDB entry 7asv coloured by chain, front view.
PDB entry 7asv coloured by chain, side view.
PDB entry 7asv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human RNA polymerase III.
Ramsay EP, Abascal-Palacios G, Daiß JL, King H, Gouge J, Pilsl M, Beuron F, Morris E, Gunkel P, Engel C, Vannini A
Nat Commun 11 6409 (2020)
PMID: 33335104

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192641 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-directed RNA polymerase III subunit RPC5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 708 amino acids
Theoretical weight: 81.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NVU0 (Residues: 1-708; Coverage: 100%)
Gene names: KIAA1452, POLR3E
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ACT 2 x ACT
2 modified residues:
Ligand MSE 42 x MSE
Ligand CME 2 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 41.11Å b: 75.76Å c: 62.4Å
α: 90° β: 103.57° γ: 90°
R-values:
R R work R free
0.18 0.18 0.21
Expression system: Escherichia coli

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Citations

12 review citations

Battle Royale: Innate Recognition of Poxviruses and Viral Immune Evasion.
Yu et al. (2021)
11 more