› 7aq4

X-ray diffraction
1.71Å resolution

Pseudomonas stutzeri nitrous oxide reductase mutant, H583E

Released:
DOI: 10.2210/pdb7aq4/pdb
PDB entry 7aq4 coloured by chain, front view.
PDB entry 7aq4 coloured by chain, side view.
PDB entry 7aq4 coloured by chain, top view.
Source organism: Pseudomonas stutzeri
Primary publication:
Histidine-Gated Proton-Coupled Electron Transfer to the CuA Site of Nitrous Oxide Reductase.
Zhang L, Bill E, Kroneck PMH, Einsle O
J Am Chem Soc 143 830-838 (2021)
PMID: 33377777

Function and Biology Details

Reaction catalysed: 
Nitrogen + H(2)O + 2 ferricytochrome c = nitrous oxide + 2 ferrocytochrome c + 2 H(+)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148648 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrous-oxide reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 646 amino acids
Theoretical weight: 71.95 KDa
Source organism: Pseudomonas stutzeri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19573 (Residues: 1-638; Coverage: 100%)
Gene name: nosZ
Sequence domains:

Ligands and Environments

10 bound ligands:
Ligand B3P 2 x B3P
Ligand NA 2 x NA
Ligand CA 2 x CA
Ligand FMT 10 x FMT
Ligand CL 2 x CL
Ligand CUA 2 x CUA
Ligand ZN 2 x ZN
Ligand TRS 1 x TRS
Ligand K 1 x K
Ligand CUZ 1 x CUZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 69.362Å b: 76.725Å c: 109.088Å
α: 90° β: 93.24° γ: 90°
R-values:
R R work R free
0.156 0.154 0.204
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

NosZ gene cloning, reduction performance and structure of Pseudomonas citronellolis WXP-4 nitrous oxide reductase.
Hu et al. (2022)
2 more