› 7rnt

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF THE TYR45TRP MUTANT OF RIBONUCLEASE T1 IN A COMPLEX WITH 2'-ADENYLIC ACID

Released:
DOI: 10.2210/pdb7rnt/pdb
PDB entry 7rnt coloured by chain, front view.
PDB entry 7rnt coloured by chain, side view.
PDB entry 7rnt coloured by chain, top view.
Source organism: Aspergillus oryzae
Primary publication:
Crystal structure of the Tyr45Trp mutant of ribonuclease T1 in a complex with 2'-adenylic acid.
Koellner G, Grunert HP, Landt O, Saenger W
Eur J Biochem 201 199-202 (1991)
PMID: 1915364

Function and Biology Details

Reaction catalysed: 
(1a) (RNA) containing guanosine = (RNA fragment)-3'-guanosine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132808 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanyl-specific ribonuclease T1 Chain: A
Molecule details ›
Chain: A
Length: 104 amino acids
Theoretical weight: 11.12 KDa
Source organism: Aspergillus oryzae
Expression system: Not provided
UniProt:
  • Canonical: P00651 (Residues: 27-130; Coverage: 100%)
Gene names: AO090011000118, rntA
Structure domains: Microbial ribonucleases

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand 2AM 1 x 2AM
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 49.32Å b: 46.61Å c: 40.92Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 not available not available
Expression system: Not provided

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Citations

3 citation in other articles

Substitution of histidine-137 by glutamine abolishes the catalytic activity of the ribosome-inactivating protein alpha-sarcin.
Lacadena et al. (1995)
2 more

1 mention without citation

Multiple structural alignment by secondary structures: algorithm and applications.
Dror et al. (2003)