› 7gat

Solution NMR

SOLUTION NMR STRUCTURE OF THE L22V MUTANT DNA BINDING DOMAIN OF AREA COMPLEXED TO A 13 BP DNA CONTAINING A TGATA SITE, 34 STRUCTURES

Released:
DOI: 10.2210/pdb7gat/pdb
PDB entry 7gat coloured by chain, ensemble of 34 models, front view.
PDB entry 7gat coloured by chain, ensemble of 34 models, side view.
PDB entry 7gat coloured by chain, ensemble of 34 models, top view.
Source organism: Aspergillus nidulans
Primary publication:
The solution structure of the Leu22-->Val mutant AREA DNA binding domain complexed with a TGATAG core element defines a role for hydrophobic packing in the determination of specificity.
Starich MR, Wikström M, Schumacher S, Arst HN, Gronenborn AM, Clore GM
J Mol Biol 277 621-34 (1998)
PMID: 9533884

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121285 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Nitrogen regulatory protein areA Chain: A
Molecule details ›
Chain: A
Length: 66 amino acids
Theoretical weight: 7.35 KDa
Source organism: Aspergillus nidulans
Expression system: Escherichia coli
UniProt:
  • Canonical: P17429 (Residues: 662-727; Coverage: 8%)
Gene names: AN8667, areA
Sequence domains: GATA zinc finger
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
DNA (5'-D(*CP*AP*GP*TP*GP*AP*TP*AP*GP*AP*GP*AP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 13 nucleotides
Theoretical weight: 4.02 KDa
Source organism: Aspergillus nidulans
Expression system: Not provided
DNA (5'-D(*GP*TP*CP*TP*CP*TP*AP*TP*CP*AP*CP*TP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 13 nucleotides
Theoretical weight: 3.92 KDa
Source organism: Aspergillus nidulans
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 review citations

The fungal GATA factors.
Scazzocchio C. (2000)
5 more

2 mentions without citation

Mechanobiology: A landscape for reinterpreting stem cell heterogeneity and regenerative potential in diseased tissues.
Tassinari et al. (2023)
1 more