› 6ydi

X-ray diffraction
1.95Å resolution

Function and Biology Details

Reaction catalysed: 
Methane + NAD(P)H + O(2) = methanol + NAD(P)(+) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-104203 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Methane monooxygenase Chain: B
Molecule details ›
Chain: B
Length: 395 amino acids
Theoretical weight: 45.3 KDa
Source organism: Methylosinus trichosporium OB3b
UniProt:
  • Canonical: A0A2D2D5X7 (Residues: 1-395; Coverage: 100%)
Gene name: CQW49_12475
Sequence domains: Methane/Phenol/Alkene Hydroxylase
Methane monooxygenase component A alpha chain Chain: D
Molecule details ›
Chain: D
Length: 526 amino acids
Theoretical weight: 60.03 KDa
Source organism: Methylosinus trichosporium OB3b
UniProt:
  • Canonical: P27353 (Residues: 1-526; Coverage: 100%)
Gene name: mmoX
Sequence domains: Methane/Phenol/Alkene Hydroxylase
Methane monooxygenase Chain: F
Molecule details ›
Chain: F
Length: 169 amino acids
Theoretical weight: 19.44 KDa
Source organism: Methylosinus trichosporium OB3b
UniProt:
  • Canonical: A0A2D2D0T0 (Residues: 1-169; Coverage: 100%)
Gene name: CQW49_12465
Sequence domains: Methane monooxygenase, hydrolase gamma chain
Methane monooxygenase regulatory protein B Chain: G
Molecule details ›
Chain: G
Length: 138 amino acids
Theoretical weight: 14.9 KDa
Source organism: Methylosinus trichosporium OB3b
Expression system: Escherichia coli
UniProt:
  • Canonical: P27356 (Residues: 1-138; Coverage: 100%)
Gene name: mmoB
Sequence domains: MmoB/DmpM family

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand FE2 2 x FE2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLAC LCLS BEAMLINE MFX
Spacegroup: P41212
Unit cell:
a: 106.946Å b: 106.946Å c: 303.989Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.175 0.197
Expression system: Escherichia coli

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