› 6uw3

X-ray diffraction
2.4Å resolution

Function and Biology Details

Reaction catalysed: 
(2S)-lactyl-2-diphospho-5'-guanosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin = GMP + factor 420-0
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106312 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoenolpyruvate transferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 327 amino acids
Theoretical weight: 34.79 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QTG2 (Residues: 1-327; Coverage: 100%)
Gene names: MSMEG_1830, MSMEI_1787, fbiA
Sequence domains: 2-phospho-L-lactate transferase CofD

Ligands and Environments

3 bound ligands:
Ligand GDP 2 x GDP
Ligand GOL 1 x GOL
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21
Unit cell:
a: 46.067Å b: 73.629Å c: 90.931Å
α: 90° β: 95.38° γ: 90°
R-values:
R R work R free
0.196 0.193 0.259
Expression system: Mycolicibacterium smegmatis MC2 155

Quick links

Citations

5 citation in other articles

Delamanid or pretomanid? A Solomonic judgement!
Mudde et al. (2022)
4 more

1 mention without citation

Cellular and Structural Basis of Synthesis of the Unique Intermediate Dehydro-F420-0 in Mycobacteria.
Grinter et al. (2020)