› 6uqq

X-ray diffraction
2.75Å resolution

Crystal Structure of GTPase Domain of Human Septin 7 / Septin 3 T282Y Heterocomplex

Released:
DOI: 10.2210/pdb6uqq/pdb
PDB entry 6uqq coloured by chain, front view.
PDB entry 6uqq coloured by chain, side view.
PDB entry 6uqq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular Recognition at Septin Interfaces: The Switches Hold the Key.
Rosa HVD, Leonardo DA, Brognara G, Brandão-Neto J, D'Muniz Pereira H, Araújo APU, Garratt RC
J Mol Biol 432 5784-5801 (2020)
PMID: 32910969

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172447 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Septin-7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 285 amino acids
Theoretical weight: 32.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q16181 (Residues: 48-318; Coverage: 62%)
Gene names: CDC10, SEPT7, SEPTIN7
Sequence domains: Septin
Neuronal-specific septin-3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 293 amino acids
Theoretical weight: 33.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UH03 (Residues: 59-337; Coverage: 78%)
Gene names: SEP3, SEPT3, SEPTIN3
Sequence domains: Septin

Ligands and Environments

1 bound ligand:
Ligand GDP 4 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P21
Unit cell:
a: 61.39Å b: 85.526Å c: 152.051Å
α: 90° β: 90.97° γ: 90°
R-values:
R R work R free
0.2 0.197 0.256
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

1 review citation

Cellular functions of actin- and microtubule-associated septins.
Spiliotis et al. (2021)
6 other articles

2 mentions without citation

The Structural Biology of Septins and Their Filaments: An Update.
Cavini et al. (2021)
1 more