› 6tb1

X-ray diffraction
1.85Å resolution

Crystal structure of thermostable omega transaminase 6-fold mutant from Pseudomonas jessenii

Released:
DOI: 10.2210/pdb6tb1/pdb
PDB entry 6tb1 coloured by chain, front view.
PDB entry 6tb1 coloured by chain, side view.
PDB entry 6tb1 coloured by chain, top view.
Source organism: Pseudomonas sp.
Primary publication:
Robust ω-Transaminases by Computational Stabilization of the Subunit Interface.
Meng Q, Capra N, Palacio CM, Lanfranchi E, Otzen M, van Schie LZ, Rozeboom HJ, Thunnissen AWH, Wijma HJ, Janssen DB
ACS Catal 10 2915-2928 (2020)
PMID: 32953233

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167496 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aspartate aminotransferase family protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 458 amino acids
Theoretical weight: 49.93 KDa
Source organism: Pseudomonas sp.
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

4 bound ligands:
Ligand PLP 2 x PLP
Ligand GOL 4 x GOL
Ligand SIN 1 x SIN
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: P43
Unit cell:
a: 98.924Å b: 98.924Å c: 120.276Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.156 0.187
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Biocatalytic Reduction Reactions from a Chemist's Perspective.
Hollmann et al. (2021)
8 other articles

2 mentions without citation

Robust ω-Transaminases by Computational Stabilization of the Subunit Interface.
Meng et al. (2020)
1 more